AF Dyes (Alexa NHS Ester) Category Fluorescent Dyes Modification Code AFDye Reference Catalog Number 26-6XXX 5 Prime Y 3 Prime Y Internal Y Molecular Weight(mw) 695.6 This modification is a post synthesis conjugation to a primary amino group thus an additional modification with an amino group is ⦠ç±å京ç¾å¥¥åæ°ç§ææéå ¬å¸æä¾ï¼äº§åç¹ç¹ï¼å京ç¾å¥¥åæ°ä»£çClick chemistry toolså¨ä¸å½çä¸å¡ãClick chemistry toolsææ代ç,Click chemistry toolså ¨å½ä»£çï¼Click chemistry toolså京代çï¼Click chemistry toolsåä¸ä»£çï¼Click chemistry toolsåå代çã 1338-1. 1 mg. $105. click chemistry tools. Description: Carboxylic acid; also available as NHS-Ester, maleimide and conjugated to streptavidin. We now offer both functionalized AF-Dyes and AF-Dye â nucleotide conjugates. 1 mg. $105. Fluoroprobes.com. engineer and combine mutually orthogonal Pyrrolysyl-tRNA-synthetase/tRNA pairs to expand the genetic code of mammalian cells with two noncanonical amino acids. The absorbance range is 346-749 nm, and the emission range is 442-775 nm. Cy7 Alkyne. CLICK AF-Dyes are routinely used for Click Chemistry â based labeling whereas NHS Ester or Maleimide-functionalized AF-Dyes react with primary amine or thiol modified biomolecules, respectively. click chemistry tools. AFDye 647 Alkyne. 1115-1. NHS-Ester, prepare the protein in phosphate-buffered saline to avoid precipitation. å ç«å¦ ç¾ç æ£æµ ç§ç æ£æµ CAS:1384870-47-6. å ¨å½å®¢æç线ï¼7*24å°æ¶ï¼ å ¨é¨äº§ååç±». è±æå称ï¼DBCO-C6-NHS Ester. AFDye 594 Alkyne. å ¨å½å®¢æç线ï¼7*24å°æ¶ï¼ å ¨é¨äº§ååç±». MB 660R Alkyne. Expert Manufacturer Years of experience synthesizing fluorescent probes, from a few milligrams to hundreds of grams Quality & Reliability - Identical Compounds - Equal Performance - Consistent Results Selection Largest selection of functional groups and dyes available off-the-shelf. Carboxyrhodamine 110 Alkyne. (C) Representative confocal microscopy images of epithelial cells expressing Muc1-42TR ÎCT showing actin staining with Alexa Fluor 568 phalloidin. 1342-1. Cy3 Alkyne. AxisPharm is a dedicated special chemical provider. I have reacted alexa fluor 647 NHS ester ⦠Mucins are labeled with Alexa Fluor 647 PNA. 1 mg. $139. NHS ester functionalized dyes were used to conjugate with secondary antibodies. Buffers that contain primary amines (e.g., Tris or glycine) will interfere because they react with the NHS-ester moiety. Afdye 647 maleimide fluoroprobes fluorescent labeling hilyteáµá´¹ fluor 647 c2 maleimide 247145 38 6 alexa fluor 568 nhs ester click chemistry tools. Recombinant His-tagged Podocalyxin was conjugated with Alexa Fluor 568 NHS Ester (Thermo Fisher Scientific), and the degree of labelling quantified according to the manufacturerâs protocol. OG 488 Alkyne. Find Alexa Dye online. AF Dyes (similar to Alexa Fluor NHS Esters) are a set of fluorescent dyes that span the visible electromagnetic spectrum, as well as some of the near-IR. The reaction mixture was protected from light and was completed in 30 min. These data repeat and elaborate on (Figures 6A and 6B). DNA is an ultra-high-density storage medium that could meet exponentially growing worldwide demand for archival data storage if DNA synthesis costs declined sufficiently and random access of files within exabyte-to-yottabyte-scale DNA data pools were feasible. Just wondering due to the stark price difference between AFDye 594 Cadaverine (Click Chemistry Tools) and Alexa Fluor 594 Cadaverine (TFS). Cy5 Alkyne. 5-TAMRA Alkyne. These two bioorthogonal chemical handles, encoded by distinct codons, allow selective, site-specific dual color fluorescent labeling and chemical-controlled crosslinking of surface receptors on living cells. å ç«å¦ ç¾ç æ£æµ ç§ç æ£æµ Cy7 NHS Ester. click chemistry tools. Trafficking of internalized NGFR/p75 receptor with p75 antibody clone 8J2, conjugated with ATTO 488 (M-1821-50-AT, green). Cells were again washed with PBS and labeled with 10 μM picolyl azide dye (AFDye 647 Picolyl Azide, Jena Bioscience), in 50 μM CuSO 4, ... (PEG 4-NHS ester)-Cy5 (N-Cy5-NHS, BroadPharm) was coupled with tetrazine amine (SiChem) at 2:1 molar ratio in PBS at 30°C for 42 h. A 200 μM stock solution was prepared in 100 mM Tris-HCl pH 8.0. 1 mg. $95. Briefly, 40 μl of secondary antibody, 5 μl of 1 M NaHCO 3, and 1 to 2 μg of fluorophore were mixed. AFDye 568 NHS Ester. We offer Alexa Fluor 647 dye conjugated to a variety of antibodies, peptides, proteins, tracers, and amplification substrates optimized for cellular labeling and detection. Home.com Domains; Fluoroprobes.com ; Fluoroprobes.com has server used 74.208.236.144 (United States) ping response time 31 ms Average ping Hosted in 1&1 IONOS Inc. Register Domain Names at 1&1 IONOS SE.This domain has been created 4 years, 276 days ago, remaining 89 days.You can check the number of websites and blacklist ip address on this server Cy5 NHS Ester. Instagram, Twitter, Facebook, Images, Youtube and more on IDCrawl - the leading free people search engine. DBCO-C6-NHS Ester. Shop a large selection of products and learn more about Click Chemistry Tools AFDye 647 NHS Ester | 25mg AFDYE 647 NHS ESTER 25MG . AFDye 488 NHS Ester. AF-Dyes (also known as Alexa Fluor® dyes) are hydrophilic fluorescent dyes with remarkable photostability. The bottom row shows the region of interest from the composite image (yellow box), n = 3. 1076-1. 1 mg. $139. For each labeling reaction, use 100-500µL of purified protein sample at 1-2.5mg/mL. TAMRA Alkyne. Meineke et al. At AxisPharm, we strive to provide our clients with special chemicals that are of the highest possible quality. AFDye 350 NHS Ester. 1336-1. Cy5.5 Alkyne. Dissolve protein directly in the labeling buffer. Alexa Fluor 647 dye molecules can be attached to proteins at high molar ratios without significant self-quenching, leading to brighter conjugates and more sensitive detection. AFDye-647 NHS ester (cat#1121-100) and sulfo-cyanine3 NHS ester (cat#1301) were purchased from Fluoroprobes. I need to buy an amine reactive dye and I was thinking to buy the alexa fluor 647 NHS ester. Atto 488 NHS ester BioReagent, suitable for fluorescence, â¥80% (coupling to amines); find Sigma-Aldrich-41698 MSDS, related peer-reviewed papers, technical documents, similar products & more at Sigma-Aldrich. ... AFDye 568 Alkyne. click chemistry tools.
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